Crystallization of the MS2 translational repressor alone and complexed to bromouridine

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Publication Type Journal Article
School or College School of Medicine; College of Science; College of Engineering
Department Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
Creator Gesteland, Raymond F.; Krapcho, Karen J.; Talbot, Phil; Thulin, Craig
Other Author Ni, Chao-Zhou; Hettinga, Bradley S.; Wickersham, John; Mitchell, Richard S.; Williamson, Michael M.; Celikel, reha; Prange, Thierry; Fourme, Roger;Ely, Kathryn R.
Title Crystallization of the MS2 translational repressor alone and complexed to bromouridine
Date 1995
Description The coat protein from the MS2 bacteriophage plays a dual role by encapsidating viral RNA and also by binding RNA as a translational repressor. In order to study the isolated dimer in a conformation not influenced by capsid interactions, a mutant molecule was crystallized that is defective in capsid assembly but is an active repressor. The unassembled dimer crystallized in the space group P21212 with a = 76.2, b = 55.7, and c = 28.4 A. In these crystals, monomers were related by twofold symmetry. When this dimer was co-crystallized with 5-bromouridine, crystals formed in space group R3 with a = b = 155.9 A, c = 29.9 A, gamma = 120 degrees; the dimer was the asymmetric unit.
Type Text
Publisher Cold Spring Harbor Lab Press
First Page 1010
Last Page 1012
Subject Crystallization; RNA Bacteriophage; RNA Hairpin; Translational Repressor
Subject MESH Crystallization; Levivirus; Point Mutation; RNA-Binding Proteins; Bromodeoxyuridine
Language eng
Bibliographic Citation Ni, C.-Z., Hettinga, B.S., Wickersham, J., Mitchell, R.S., Williamson, M.M., Celikel, R., Prange, T., Fourme, R., Krapcho, K.J., Thulin, C., Talbot, P., Gesteland, R.F. and Ely, K. 1995. Crystallization of the MS2 translational repressor alone and complexed to bromouridine. Protein Sci. 4:1010-1012
Rights Management © Cold Spring Harbor Laboratory Press-CSHL Press
Format Medium application/pdf
Identifier ir-main,12769
ARK ark:/87278/s6f486mh
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 705703
Reference URL https://collections.lib.utah.edu/ark:/87278/s6f486mh
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