Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassay

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Publication Type Journal Article
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Jaskowski, Troy D.
Other Author Martins, Thomas B.; Pasi, Brian M.; Pickering, Jerry W.; Litwin, Christine M.; Hill, Harry R.
Title Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassay
Date 2002
Description We used a multiplexed fluorescent microsphere immunoassay to develop a sandwich capture assay to assess simultaneously the production of thymus helper (TH) 1- and TH2-type cytokines in tissue culture supernatant obtained from stimulated peripheral blood mononuclear cells. The assay then was used to assess the cytokine production of patients with hyperimmunoglobulinemia E syndrome and in cord blood from neonates. The multiplexed assay has a reportable range of less than 10 to 50,000 pg/mL. For linearity and recovery studies, R2 values for the 6 cytokines ranged from 0.988 to 0.999 for samples spiked with known concentrations of recombinant cytokine standards and for patient samples. The assay showed good specificity, with little cross-reactivity between cytokines. Results from supernatants of Staphylococcus aureus-stimulated peripheral blood mononuclear cells obtained from 6 patients with hyperimmunoglobulinemia E syndrome showed significantly less interferon (IFN)-gamma production than cells from healthy control subjects. Cord blood cells from neonates produced significantly less interleukin 12 and IFN-gamma than cells from adults in group B streptococci-stimulated mononuclear cells. The fluorescent multiplexed microsphere immunoassay can be used to quantitate multiple cytokines from 1 sample and should be useful for further understanding of the cytokine role in disease.
Type Text
Publisher American Society for Clinical Pathology
Volume 118
Issue 3
First Page 346
Last Page 353
Subject Multiplexed fluorescent microsphere immunoassay
Subject LCSH Cytokines; Immunoassay; Immunodeficiency
Language eng
Bibliographic Citation Martins, T. B., Pasi, B. M., Pickering, J. W., Jaskowski, T. D., Litwin, C. M., & Hill, H. R. (2002). Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassay. American Journal of Clinical Pathology, 118(3), 346-53.
Rights Management ©1996-2010 American Society for Clinical Pathology and ©1996-2010 ASCP Press
Format Medium application/pdf
Format Extent 112,482 bytes
Identifier ir-main,11448
ARK ark:/87278/s6k36c4f
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 705641
Reference URL https://collections.lib.utah.edu/ark:/87278/s6k36c4f
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