Role of photoreceptor-specific retinol dehyrogenase (prRDH) in the retinoid cycle in vivo

Update Item Information
Publication Type Journal Article
School or College School of Medicine
Department Ophthalmology
Creator Baehr, Wolfgang
Other Author Maeda, Akiko; Maeda, Tadao; Imanishi, Yoshikazu; Kudsa, Vladimir; Alekseev, Andrei; Bronson, Darin J.; Zhang, Houbin; Zhui, Li; Sun, Wenyu; Saperstein, David A.; Rieke, Fred; Palczewski, Krzysztof
Title Role of photoreceptor-specific retinol dehyrogenase (prRDH) in the retinoid cycle in vivo
Date 2005
Description The retinoid cycle is a recycling system that replenishes the 11-cis-retinal chromophore of rhodopsin and cone pigments. Photoreceptor-specific retinol dehydrogenase (prRDH) catalyzes reduction of all-trans-retinal to all-trans-retinol and is thought to be a key enzyme in the retinoid cycle. We disrupted mouse prRDH (human gene symbol RDH8) gene expression by targeted recombination and generated a homozygous prRDH knockout (prRDH-/-) mouse. Histological analysis and electron microscopy of retinas from 6-8 week-old prRDH-/- mice revealed no structural differences of the photoreceptors or inner retina. For brief light exposure, absence of prRDH did not affect the rate of 11-cis-retinal regeneration or the decay of Meta II, the activated form of rhodopsin. Absence of prRDH did, however, cause significant accumulation of all-trans-retinal following exposure to bright lights, and delayed recovery of rod function as measured by electroretinograms and single cell recordings. Retention of all-trans-retinal resulted in slight overproduction of A2E, a condensation product of all-trans-retinal and phosphatidylethanolamine. We conclude that prRDH is an enzyme that catalyzes reduction of all-trans-retinal in the rod outer segment, most noticeably at higher light intensities and prolonged illumination, but is not an essential enzyme of the retinoid cycle.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Subject Rhodopsin, Photoreceptors; prRDH (RDH8); All-trans-RDH; A2E; Retinoid cycle; Dark adaptation
Subject MESH Rhodopsin; Eye; Photoreceptors, Vertebrate
Language eng
Bibliographic Citation Maeda A, Maeda T, Imanishi Y, Kuksa V, Alekseev A, Bronson DJ, Zhang H, Zhu L, Sun W, Saperstein DA, Rieke F, Baehr W, Palczewski K. (2005). Role of photoreceptor-specific retinol dehyrogenase (prRDH) in the retinoid cycle in vivo. J Biol Chem
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Identifier ir-main,256
ARK ark:/87278/s6sj242g
Setname ir_uspace
ID 705471
Reference URL https://collections.lib.utah.edu/ark:/87278/s6sj242g
Back to Search Results