Hepatitis C genotyping by denaturing high-performance liquid chromatography

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Publication Type Journal Article
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Liew, Michael; Erali, Maria; Page, Sam; Hillyard, David R.; Wittwer, Carl T.
Title Hepatitis C genotyping by denaturing high-performance liquid chromatography
Date 2004-01
Description Determination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 11 major genotypes and >70 subtypes, contributes to the technical difficulty of genotype testing. While the "gold standard" for testing is nucleic acid sequencing, a variety of hybridization assays, including the line probe assay, have been developed to provide more rapid and accessible forms of testing. The aim of this study was to determine whether denaturing high-performance liquid chromatography (dHPLC) could be used as a clinical method for distinguishing HCV genotypes 1, 2, 3, and 4. A portion of the 5' untranslated region of the HCV genome was amplified by heminested multiplex reverse transcription PCR. The two amplicons then were analyzed by dHPLC analysis and compared to the genotypes determined by sequence analysis. After 115 specimens were analyzed as standards, 200 masked specimens (specimens whose identity was not known before testing) were analyzed to determine the concordance of the assay. The assay had a concordance of 96% at the genotype level and a concordance of 87% at the subtype level. However, the dHPLC method was not as accurate as other reported methods of HCV genotyping. This is the first time that HCV genotyping has been performed by dHPLC.
Type Text
Publisher American Society for Microbiology
Volume 42
Issue 1
First Page 158
Last Page 163
Subject Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data
Subject MESH Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data
Language eng
Bibliographic Citation Liew, M., Erali, M., Page, S., Hillyard, D., & Wittwer, C.T. (2004). Hepatitis C genotyping by denaturing high-performance liquid chromatography. Journal of Clinical Microbiology, 42(1), 158-163. DOI: 10.1128/JCM.42.1.158-163.2004
Rights Management (c)2004 by the American Society for Microbiology. http://www.journals.asm.org/misc/terms.shtmlliquid>http://www.journals.asm.org/misc/terms.shtml
Format Medium application/pdf
Format Extent 449,015 bytes
Identifier ir-main,156
ARK ark:/87278/s6tt58bx
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 705402
Reference URL https://collections.lib.utah.edu/ark:/87278/s6tt58bx
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