Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responses

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Publication Type Journal Article
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Jaskowski, Troy D.
Other Author Mouritsen, C. Lars; Wittwer, Carl T.; Litwin, Christine M.; Yang, Liming; Weis, Janis J.; Martins, Thomas B.; Hill, Harry R.
Title Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responses
Date 1996
Description The Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme disease in humans. With denaturing and annealing temperature spikes instead of holds, cycle times were less than 20 minutes for a 30-cycle amplification. Using this rapid cycle PCR technique, as few as 5 spirochetes per mL of phosphate buffered saline were detected. In addition, B burgdorferi DNA was detected from spirochetes that had been spiked into one of several types of human body fluids including serum, synovial fluid, and cerebrospinal fluid (CSF). A number o f clinical samples, which had been tested for Lyme immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody were also examined.
Type Text
Publisher American Society for Clinical Pathology
Volume 105
Issue 5
First Page 647
Last Page 654
Subject PCR; Rapid cycle amplification
Subject LCSH Polymerase chain reaction; Lyme disease; Borrelia burgdorferi; Lyme disease -- Diagnosis
Language eng
Bibliographic Citation Mouritsen, C. L., Wittwer, C. T., Litwin, C. M., Yang, L., Weis, J. J., Martins, T. B., Jaskowski, T. D., & Hill, H. R. (1996). Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responses. American Journal of Clinical Pathology, 105(5), 647-54.
Rights Management ?1996-2010 American Society for Clinical Pathology and ?1996-2010 ASCP Press
Format Medium application/pdf
Format Extent 949,023 bytes
Identifier ir-main,11460
ARK ark:/87278/s6fn1qnp
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 705203
Reference URL https://collections.lib.utah.edu/ark:/87278/s6fn1qnp
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