Cross platform comparison of melting curve analysis

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Publication Type Poster
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V.
Title Cross platform comparison of melting curve analysis
Date 2005-11-04
Description Melting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melting analysis for genotyping and screening. With the gaining acceptance of melting analysis as a diagnostic tool, there is a need to characterize the ability of commercially available real-time PCR instruments to perform melting analyses. In the current study, a systematic evaluation of melting analysis capability was undertaken employing eight real-time machines from six vendors in resolving SNP genotypes by melting with SYBR Green I and LCGreen Plus.
Type Text
Publisher Associated and Regional University Pathologists (ARUP) Institute for Clinical and Experimental Pathology
Subject PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping
Subject MESH Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Genotype; Genetic Techniques
Language eng
Rights Management
Format Medium application/pdf
Format Extent 4,335,388 bytes (4.13 MB)
Identifier ir-main,12
ARK ark:/87278/s6q24hmh
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 705025
Reference URL
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