Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Internal Medicine; Oncological Sciences; Human Genetics; Biology
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Thomas, Kirk R.; Capecchi, Mario R.
Title Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene.
Date 1986-11-06
Description Injection of homologous DNA sequences into nuclei of cultured mammalian cells induces mutations in the cognate chromosomal gene. It appears that these mutations result from incorrect repair of a heteroduplex formed between the introduced and the chromosomal sequence. This phenomenon is termed 'heteroduplex induced mutagenesis'. The high frequency of these events suggests that this method may prove useful for introducing mutations into specific mammalian genes.
Type Text
Publisher Nature Publishing Group
Volume 324
Issue 6092
First Page 34
Last Page 38
Subject Animals; Cell Line; Drug Resistance, Microbial; Fibroblasts; Mice; Models, Genetic; Neomycin; Plasmids
Subject MESH DNA, Recombinant; Mutation; Protein Biosynthesis; Sequence Homology, Nucleic Acid
Language eng
Bibliographic Citation Nature. 1986 Nov 6-12;324(6092):34-8. Thomas KR, Capecchi MR. Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene. Retrieved on November 2, 2006 from http://www.nature.com/nature/journal/v324/n6092/abs/324034a0.html;jsessionid=0E0CC7587E52658B6938C16BDD67C5C4.
Rights Management copyright © 1986 Nature Publishing Group. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,554
ARK ark:/87278/s6w6741d
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 704159
Reference URL https://collections.lib.utah.edu/ark:/87278/s6w6741d
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