Metabolism and transactivation activity of 13,14-dihydroretinoic acid

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Publication Type Journal Article
School or College School of Medicine
Department Ophthalmology
Creator Baehr, Wolfgang
Other Author Moise, Alexander R;Kuksa, Vladimir; Blaner, William S; Palczewski, Krzysztof
Title Metabolism and transactivation activity of 13,14-dihydroretinoic acid
Date 2005
Description The metabolism of vitamin A is a highly regulated process that generates essential mediators involved in the development, cellular differentiation, immunity, and vision of vertebrates. Retinol saturase converts all-trans-retinol to all-trans-13,14-dihydroretinol (Moise, A. R., Kuksa, V., Imanishi, Y., and Palczewski, K. (2004) J. Biol. Chem. 279, 50230-50242). Here we demonstrate that the enzymes involved in oxidation of retinol to retinoic acid and then to oxidized retinoic acid metabolites are also involved in the synthesis and oxidation of all-trans-13,14-dihydroretinoic acid. All-trans-13,14-dihydroretinoic acid can activate retinoic acid receptor/retinoid X receptor heterodimers but not retinoid X receptor homodimers in reporter cell assays. All-trans-13,14-dihydroretinoic acid was detected in vivo in Lrat-/- mice supplemented with retinyl palmitate. Thus, all-trans-13,14-dihydroretinoic acid is a naturally occurring retinoid and a potential ligand for nuclear receptors. This new metabolite can also be an intermediate in a retinol degradation pathway or it can serve as a precursor for the synthesis of bioactive 13,14-dihydroretinoid metabolites.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 280
Issue 30
First Page 27815
Last Page 27825
Subject Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Retinoid X Receptors
Subject MESH Cytochrome P-450 Enzyme System; Tretinoin; Trans-Activation (Genetics)
Language eng
Bibliographic Citation Moise AR, Kuksa V, Blaner WS, Baehr W, Palczewski K. (2005). Metabolism and transactivation activity of 13,14-dihydroretinoic acid. J Biol Chem, 280(30), 27815-25
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Identifier ir-main,1743
ARK ark:/87278/s60s06pw
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 704013
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