Altered enzymes in drug-resistant variants of mammalian tissue culture cells.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Sharp, J. D.; Capecchi, Nancy E
Title Altered enzymes in drug-resistant variants of mammalian tissue culture cells.
Date 1973-11
Description Two selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in theenzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8.) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variantscan arise from structural gene mutations.
Type Text
Publisher National Academy of Sciences of the United States of America.
Volume 70
Issue 11
First Page 3145
Last Page 3149
Subject Drug Resistance; Azaguanine; Clone Cells; Hypoxanthines
Subject MESH Genes; Mutation; Pentosyltransferases; Nitrosoguanidines
Language eng
Bibliographic Citation Proc Natl Acad Sci U S A. 1973 Nov;70(11):3145-9: Sharp JD, Capecchi NE, Capecchi MR, Altered enzymes in drug-resistant variants of mammalian tissue culture cells. Retrieved on September 15.2006 from http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=4522295.
Rights Management Copyright 1973 National Academy of Sciences of the United States of America. All right Reserved
Format Medium application/pdf
Identifier ir-main,423
ARK ark:/87278/s6p27gd7
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 703888
Reference URL https://collections.lib.utah.edu/ark:/87278/s6p27gd7
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