Processing of adenovirus 2-induced proteins

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Publication Type Journal Article
School or College School of Medicine; College of Science
Department Bioengineering; Biology; Human Genetics
Creator Gesteland, Raymond F.
Other Author Baum, Peter R.; Anderson, Carl W.
Title Processing of adenovirus 2-induced proteins
Date 1973
Description Analysis of (35)S-methionine-labeled extracts of adenovirus 2-infected KB cells revealed 22 virus-induced polypeptide components. Most proteins of the virion were easily detected in extracts of whole cells labeled for short periods between 15 and 30 h after infection; however, several virion components were conspicuously absent. Radioactivity appeared in two of these virion components during a chase in nonradioactive medium, and this appearance was paralleled by a decrease in the radioactivity associated with two nonvirion adenovirus-induced proteins, results which imply precursor-product relationships for these components. Comparison of one of the chasable adenovirus-induced components (designated P-VII; mass of 20,000 daltons) and the major core protein (VII; mass of 18,500 daltons) of the virion showed that they have four common methionine-containing tryptic peptides; P-VII has an additional methionine residue which is not found in the major core protein. We propose that at least two of the adenovirus 2 virion components are derived by the cleavage of higher molecular weight precursor polypeptides.
Type Text
Publisher American Society for Microbiology
Volume 12
Issue 2
First Page 241
Last Page 252
Subject Adenoviridae; Viral Proteins; Protein Precursors; Methionine; Mouth Neoplasms
Subject MESH Adenoviridae; Methionine; Peptides; Virus Replication; Sulfur Radioisotopes; Methionine
Language eng
Bibliographic Citation Anderson, C. W., Baum, P. R., & Gesteland, R. F. (1973). Processing of adenovirus 2-induced proteins. Journal of Virology, 12(2), 241-52.
Rights Management ©American Society for Microbiology
Format Medium application/pdf
Identifier ir-main,12741
ARK ark:/87278/s6tt585p
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 703675
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