Influence of the stacking potential of the base 3' of tandem shift codons on -1 ribosomal frameshifting used for gene expression

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Publication Type Journal Article
School or College School of Medicine
Department Human Genetics
Creator Gesteland, Raymond F.; Atkins, John F.
Other Author Bertrand, Claire; Prere, Marie Francoise; Fayet, Olivier
Title Influence of the stacking potential of the base 3' of tandem shift codons on -1 ribosomal frameshifting used for gene expression
Date 2002
Description Translating ribosomes can shift reading frame at specific sites with high efficiency for gene expression purposes. The most common type of shift to the 1 frame involves a tandem realignment of two anticodons from pairing with mRNA sequence of the form X XXY YYZ to XXX YYY Z where the spaces indicate the reading frame. The predominant 1 shift site of this type in eubacteria is A AAA AAG. The present work shows that in Escherichia coli the identity of the 6 nt 39 of this sequence can be responsible for a 14-fold variation in frameshift frequency. The first 39 nucleotide has the primary effect, with, in order of decreasing efficiency, U . C . A . G. This effect is independent of other stimulators of frameshifting. It is detected with other X XXA AAG sequences, but not with several other heptameric 1 shift sites. Pairing of E. coli tRNALys with AAG is especially weak at the third codon position. We propose that strong stacking of purines 39 of AAG stabilizes pairing of tRNALys, diminishing the chance of codon:anticodon dissociation that is a prerequisite for the realignment involved in frameshifting.
Type Text
Publisher Cold Spring Harbor Lab Press
Volume 8
Issue 1
First Page 16
Last Page 28
Subject 1 frameshifting; 39 context effect; Codon anticodon interaction; Escherichia coli ; Recoding; tRNALys; XXXY YYZ frameshift motifs
Subject MESH Gene Expression Regulation, Bacterial; Eubacterium
Language eng
Bibliographic Citation Bertrand C, Prère MF, Gesteland RF, Atkins JF, Fayet O. 2002. Influence of the stacking potential of the base 3' of tandem shift codons on -1 ribosomal frameshifting used for gene expression. RNA 8(1):16-28
Rights Management (c) Cold Spring Harbor Laboratory Press-CSHL Press
Format Medium application/pdf
Format Extent 821,788 Bytes
Identifier ir-main,3797
ARK ark:/87278/s6q81x9c
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 703645
Reference URL https://collections.lib.utah.edu/ark:/87278/s6q81x9c
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