Genetic interaction between hoxb-5 and hoxb-6 is revealed by nonallelic noncomplementation.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Rancourt, Derrick R.; Tsuzuki, Teruhisa
Title Genetic interaction between hoxb-5 and hoxb-6 is revealed by nonallelic noncomplementation.
Date 1995-01-01
Description hoxb-5 and hoxb-6 are adjacent genes in the mouse HoxB locus and are members of the homeotic transcription factor complex that governs establishment of the mammalian body plan. To determine the roles of these genes during development, we generated mice with a targeted disruption in each gene. Three phenotypes affecting brachiocervicothoracic structures were found in the mutant mice. First, hoxb-5- homozygotes have a rostral shift of the shoulder girdle, analogous to what is seen in the human Sprengel anomaly. This suggests a role for hoxb-5 in specifying the position of limbs along the anteroposterior axis of the vertebrate body. Second, hoxb-6- homozygotes frequently have a missing first rib and a bifid second rib. The third phenotype, an anteriorizing homeotic transformation of the cervicothoracic vertebrae from C6 through T1, is common to both hoxb-5- and hoxb-6- homozygotes. Quite unexpectedly, hoxb-5, hoxb-6 transheterozygotes (hoxb-5-hoxb-6+/hoxb-5+ hoxb-6-) also show the third phenotype. By this classical genetic complementation test, these two mutations appear as alleles of the same gene. This phenomenon is termed nonallelic noncomplementation and suggests that these two genes function together to specify this region of the mammalian vertebral
Type Text
Publisher Cold Spring Harbor Lab Press
Volume 9
Issue 1
First Page 108
Last Page 122
Subject Alleles; CCAAT-Enhancer-Binding Proteins; Cervical Vertebrae; DNA-Binding Proteins; Forelimb; Genetic Complementation Test
Subject MESH Bone and Bones; Genes, Homeobox; Homeodomain Proteins; Mice, Mutant Strains
Language eng
Bibliographic Citation Genes Dev. 1995 Jan 1;9(1):108-22: Rancourt DR, Tsuzuki T, Capecchi MR. Genetic interaction between hoxb-5 and hoxb-6 is revealed by nonallelic noncomplementation. Retrieved on September 25.2006 from
Rights Management Copyright © 1995 Cold Spring Harbor Laboratory Press. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,468
ARK ark:/87278/s6m33cxx
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 703523
Reference URL https://collections.lib.utah.edu/ark:/87278/s6m33cxx
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