Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Spyropoulos, Demetri D.
Title Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus.
Date 1994-08-15
Description Implantation within the mammalian uterus elicits dramatic changes in the growth, differentiation, and morphogenesis of the conceptus. This process is interrupted in mice carrying a targeted disruption of the murine evx1 gene, a homolog of the Drosophila even-skipped (eve) gene. Upon implantation, presumptive evx1- homozygotes elicit a decidual response, invade the uterine epithelium, and attach to the basement membrane between uterine stroma and epithelium, but fail to differentiate extraembryonic tissues or to form egg cylinders prior to resorption. Retrograde analysis of embryo genotypes demonstrates that homozygotes could be isolated as free-floating blastocysts but not as gastrulating egg cylinders. Homozygous mutant blastocysts appeared normal and, when grown in vitro, attach, proliferate, and form trophoblastic giant cells surrounding a growing inner cell mass before rapidly degenerating. In situ hybridization analysis demonstrates evx1 gene expression within the visceral endoderm after implantation and prior to gastrulation, at a time in which the mutant phenotype is first detected.
Type Text
Publisher Cold Spring Harbor Lab Press
Volume 8
Issue 16
First Page 1949
Last Page 1961
Subject Extraembryonic; Homozygotes; Drosophila
Subject MESH Embryonic and Fetal Development; Embryo Implantation; Genes, Homeobox; Molecular Sequence Data
Language eng
Bibliographic Citation Spyropoulos DD, Capecchi MR. Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus. Genes Dev. 1994 Aug 15;8(16):1949-61.
Format Medium application/pdf
Identifier ir-main,367
ARK ark:/87278/s6sf3dc5
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 703394
Reference URL https://collections.lib.utah.edu/ark:/87278/s6sf3dc5
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