Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay.

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Publication Type Poster
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.
Title Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay.
Date 2004-05-10
Description To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international standard Metods: To evaluate linearity, dilutions of an Armored RNA solution and high titer patient specimens assayed in the Alliance assay using the Qiagen 9604 BioRobot and QIAamp Virus BioRobot 9604 Kit for RNA extraction. A split sample study was conducted using one hundred forty clilnical samples previously assayed by CTM assay (calibrated directly to the 2nd WHO international standard, with results from 200 to 100.000,000 IU/mL) and tested in the Alliance assay. The results were analyzed using Deming regression. Ninety-two clinical samples previously tested in the CTM assay with result <200 IU/mL were randomly selected and tested in the Alliance assay to determine the number of samples were measurable HCV RNA status. Within-run linearity was evaluated using replicated date from the linerity studies and between-run precisoion was estimated from control material replicated.
Type Text
Publisher University of Utah
Subject Quantitative Tests; Linearity Studies
Subject MESH Clinical Laboratory Techniques; Laboratory Techniques and Procedures; Chemistry, Clinical; Evaluation Studies
Language eng
Bibliographic Citation Konnick EQ, Ashwood ER, Hillyard DR. Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay.
Format Medium application/pdf
Identifier ir-main,515
ARK ark:/87278/s6jd5f1b
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 703303
Reference URL https://collections.lib.utah.edu/ark:/87278/s6jd5f1b
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