|School or College
|School of Medicine; College of Science; College of Engineering
|Human Genetics; Howard Hughes Medical Institute; Bioengineering; Biology
|Gesteland, Raymond F.; Weiss, Robert B.
|Wagner, Lori A.; Dayhuff, Timothy J.
|Efficient shine- Dalgarno sequence but not translation is necessary for LacZ mRNA stability in Escherichia coli
|The 5' ends of many bacterial transcripts are important in determining mRNA stability. A series of Shine-Dalgarno (SD) sequence changes showed that the complementarity of the SD sequence to the anti-SD sequence of 16S rRNA correlates with lacZ mRNA stability in Escherichia coli. Several initiation codon changes showed that an efficient initiation codon is not necessary to maintain lacZ mRNA stability. A stop codon in the 10th codon of lacZ increased mRNA stability. Therefore, ribosomal binding via the SD sequence but not translation of the coding region is necessary to maintain lacZ mRNA stability.
|American Society for Microbiology
|Protein Biosynthesis; Ribosomes; Escherichia coli; Shine-Dalgarno Sequence
|Molecular Sequence Data; Mutation; Protein Biosynthesis
|Wagner, L.A., Gesteland, R.F., Dayhuff, T.J. and Weiss, R.B. 1994. An efficient Shine- Dalgarno sequence but not translation is necessary for LacZ mRNA stability in Escherichia coli. J. Bact. 176:1683-1688
|©American Society for Microbiology