Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis

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Publication Type Abstract
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M.
Title Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis
Date 2006-01-09
Description Differentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar nucleotide sequences, a rapid, reliable method to distinguish species within MTC is not widely available. Genomic deletion analysis has been used, but is labor-intensive. We developed a genomic deletion assay based on real-time PCR with melting temperature (Tm) analysis to differentiate species within MTC. Conclusions: Melting analysis of RD 1, 9, and 10 can identify Mtb, Mbcg, and Ma. Development of multiplex real-time PCR with Tm analysis and additional primers to differentiate other species within MTC warrants further study and is ongoing.
Type Text
Publisher Associated and Regional University Pathologists (ARUP) Institute for Clinical and Experimental Pathology
Subject MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR
Subject LCSH Mycobacterium tuberculosis; Species; Mycobacterium bovis; Polymerase chain reaction
Subject MESH Genetic Speciation; Mycobacterium tuberculosis; Mycobacterium bovis; Polymerase Chain Reaction
Language eng
Rights Management http://creativecommons.org/licenses/by-nc-nd/2.5/
Format Medium application/pdf
Format Extent 63,080 bytes (961.6 KB)
Identifier ehsl-arup,19
ARK ark:/87278/s6hh737c
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 703105
Reference URL https://collections.lib.utah.edu/ark:/87278/s6hh737c
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