Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand

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Publication Type Journal Article
School or College School of Medicine
Department Human Genetics
Program Howard Hughes Medical Institute (HHMI)
Creator Gesteland, Raymond F.; Letsou, Anthea; Ivanov, Ivaylo P.; Simin, Karl; Atkins, John F.
Title Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand
Date 1998
Description Previously, a Drosophila melanogaster sequence with high homology to the sequence for mammalian antizyme (ornithine decarboxylase antizyme) was reported. The present study shows that homology of this coding sequence to its mammalian antizyme counterpart also extends to a 5* open reading frame (ORF) which encodes the amino-terminal part of antizyme and overlaps the 11 frame (ORF2) that encodes the carboxy-terminal three-quarters of the protein. Ribosomes shift frame from the 5* ORF to ORF2 with an efficiency regulated by polyamines. At least in mammals, this is part of an autoregulatory circuit. The shift site and 23 of 25 of the flanking nucleotides which are likely important for efficient frameshifting are identical to their mammalian homologs. In the reverse orientation, within one of the introns of the Drosophila antizyme gene, the gene for snRNP Sm D3 is located. Previously, it was shown that two closely linked P-element transposon insertions caused the gutfeeling phenotype of embryonic lethality and aberrant neuronal and muscle cell differentiation. The present work shows that defects in either snRNP Sm D3 or antizyme, or both, are likely causes of the phenotype.
Type Text
Publisher American Society for Microbiology
First Page 1553
Last Page 1561
Subject Ornithine decarboxylase antizyme; Binding proteins
Subject LCSH Notch genes; Genetics
Language eng
Bibliographic Citation Ivanov, I.P., Simin, K., Letsou, A., Atkins, J.F., and Gesteland, R.F. 1998. The Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand. Mol. Cell. Biol. 18:1553-1561
Rights Management (c)American Society for Microbiology
Format Medium application/pdf
Format Extent 323,726 Bytes
Identifier http://content.lib.utah.edu/u?/ir-main,12776
ARK ark:/87278/s6931b9w
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 703068
Reference URL https://collections.lib.utah.edu/ark:/87278/s6931b9w
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