Measurement of suppressor transfer RNA activity.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Young, James F.; Laski, Frank A.; RajBhandary, Uttam L.; Sharp PA; Palese P
Title Measurement of suppressor transfer RNA activity.
Date 1983-08-26
Description Transfer RNA (tRNA) suppression of nonsense mutations in prokaryotic systems has been widely used to study the structure and function of different prokaryotic genes. Through genetic engineering techniques, it is now possible to introduce suppressor (Su+) tRNA molecules into mammalian cells. A quantitative assay of the suppressor tRNA activity in these mammalian cells is described; it is based on the amount of tRNA-mediated readthrough of a terminating codon in the influenza virus NS1 gene after the cells are infected with virus. Suppressor activity in L cells continuously expressing Su+ (tRNAtyr) was 3.5 percent and that in CV-1 cells infected with an SV40- Su+ (tRNAtyr) recombinant was 22.5 percent.
Type Text
Publisher American Association for the Advancement of Science (AAAS)
Volume 221
Issue 4613
First Page 873
Last Page 875
Subject Animals; Cells, Cultured; Eukaryotic Cells; Genes, Viral; Mice; Orthomyxoviridae; Peptide Chain Termination, Translational; Protein Biosynthesis
Subject MESH RNA, Transfer; Suppression, Genetic
Language eng
Bibliographic Citation Science. 1983 Aug 26;221(4613):873-5: Young JF, Capecchi MR, Laski FA,RajBhandary UL, Sharp PA, Palese P. Measurement of suppressor transfer RNA activity. Retrieved on September 18.2006 from
Rights Management Copyright © 1983 American Association for the Advancement of Science. All Rights Reserved..
Format Medium application/pdf
Identifier ir-main,475
ARK ark:/87278/s62522bx
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 702772
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