Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis

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Publication Type Poster
School or College School of Medicine
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T.
Title Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis
Date 2005-06
Description This poster describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the method allows rapid development of low-cost assays and does not require any arrays or complex instrumentation.
Type Text
Publisher Associated and Regional University Pathologists (ARUP) Institute for Clinical and Experimental Pathology
Subject Genotyping; Amplicon Melting
Subject MESH Genetic Techniques; Genotype
Language eng
Rights Management http://creativecommons.org/licenses/by-nc-nd/2.5/
Format Medium application/pdf
Format Extent 3,608,995 bytes (3.44 MB)
Identifier ir-main,78
ARK ark:/87278/s6jd5dw4
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 702334
Reference URL https://collections.lib.utah.edu/ark:/87278/s6jd5dw4
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