Transepithelial transport of pamam dendrimers across isolated intestinal tissue

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Publication Type dissertation
School or College College of Engineering
Department Bioengineering
Author Hubbard, Dallin Andrew
Title Transepithelial transport of pamam dendrimers across isolated intestinal tissue
Date 2016-05
Description Poly(amido amine) (PAMAM) dendrimers have shown potential to carry poorly absorbed drugs across the intestinal barrier and into systemic circulation, reducing the need for intravenous injections. Much of the in vitro transepithelial transport of PAMAM dendrimers to date has been investigated using Caco-2 monolayers which lack the microvilli morphology and enzymes present in isolated intestinal tissues. In addition, a challenge in predicting oral absorption is establishing a correlation between transport across rodent and human intestinal tissues. This dissertation focused on investigating the transepithelial transport of PAMAM dendrimers across rat and human isolated intestinal tissues. Permeability values in isolated tissues were compared with those across Caco-2 cell monolayers. Results indicate a difference in transport of PAMAM dendrimers, morphological changes and transepithelial electrical resistance between Caco-2 cell monolayers, rat and human intestinal tissue models. A relatively high transport rate across the tissues, given the macromolecular nature of PAMAM dendrimers, shows promise for use of these constructs for oral delivery in human.
Type Text
Publisher University of Utah
Subject dendrimer; drug delivery; oral delivery; Ussing
Dissertation Institution University of Utah
Dissertation Name Doctor of Philosophy
Language eng
Rights Management Copyright © Dallin Andrew Hubbard 2016
Format Medium application/pdf
Format Extent 26,534 bytes
Identifier etd3/id/4102
ARK ark:/87278/s6351tpf
Setname ir_etd
Date Created 2016-08-25
Date Modified 2017-09-11
ID 197652
Reference URL https://collections.lib.utah.edu/ark:/87278/s6351tpf