Optic Tract Formation: Retinal Axons' Interaction with Their Pioneers and Peers, and a Strategy to Analyze Variants of Guidance Receptor Messengers RNAs

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Publication Type dissertation
School or College School of Medicine
Department Interdepartmental Program in Neuroscience
Author Law, Mei Yee
Title Optic Tract Formation: Retinal Axons' Interaction with Their Pioneers and Peers, and a Strategy to Analyze Variants of Guidance Receptor Messengers RNAs
Date 2011-12
Description How neural connections are established spontaneously to generate a functioning nervous system remains an important question in the field of developmental biology. Axons and dendrites extended from various cell types have to form at the right place and right time to make appropriate connections. An enormous number of molecules is present in the developing embryos. Experimental analyses are further complicated by virtue of these molecules' ability to interact and affect the function of other molecules. I first set out to test the role of a cell biological mechanism-axon fasciculation-in guiding axon pathfinding within the zebrafish visual system. I then focused on the role of a transmembrane receptor, Robo2, and its splice variants in axon guidance. In Chapter 1, I present some important questions in axon guidance and alternative splicing, along with reviews of factors involved in retinal axon guidance. I also introduce the roles of alternative splicing in increasing protein diversity and thus contributing to various responses in pathfinding along the axon trajectory. Chapter 2 is a collaborative peer-reviewed paper published in Development showing that early-born retinal ganglion cells guide later-born cells within and outside of the eye. In Chapter 3, I described my observation of robo2 alternative splice forms in developing zebrafish embryos and my experiments using morpholinos to test the domains iv functionally. Finally, Chapter 4 discusses the findings and future directions of the studies.
Type Text
Publisher University of Utah
Subject MESH Axons; Retina; Morphogenesis; Receptors, Cell Surface; Growth Cones; Retinal Ganglion Cells; Intercellular Signaling Peptides and Proteins; Signal Transduction; Semaphorins; Cell Adhesion Molecules, Neuron-Glia; Receptors, Presynaptic; Gene Expression Regulation, Developmental; Zebrafish
Dissertation Institution University of Utah
Dissertation Name Doctor of Philosophy
Language eng
Relation is Version of Digital reproduction of Optic Tract Formation: Retinal Axons' Interaction with Their Pioneers and Peers, and a Strategy to Analyze Variants of Guidance Receptor Messengers RNAs
Rights Management Copyright © Mei Yee Law 2011
Format Medium application/pdf
Format Extent 86,991,803 bytes
Source Original in Marriott Library Special Collections
ARK ark:/87278/s6f50xcc
Setname ir_etd
Date Created 2014-06-09
Date Modified 2019-11-05
ID 196511
Reference URL https://collections.lib.utah.edu/ark:/87278/s6f50xcc
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