An engineering approach to direct and characterize the structure of cardiac tissue

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Publication Type dissertation
School or College College of Engineering
Department Bioengineering
Author Lasher, Richard Allen
Title An engineering approach to direct and characterize the structure of cardiac tissue
Date 2012
Description Treatment and management of heart disease is challenging due to the heart's limited ability to self-repair. Although current approaches to manage heart disease, such as pharmacotherapy, medical devices, lifestyle changes, and heart transplantation, have improved and extended the quality of life for millions of individuals, they have inherent shortcomings. Future strategies to manage heart disease will likely be based upon a combination of biological and engineering approaches through cell therapy and tissue engineering strategies, both of which have the potential to regenerate the myocardium and improve cardiac function. However, a key hurdle in applying biological approaches is our limited ability to produce reliable tissue to study disease progression and tissue development, therapeutic intervention, drug discovery, or tissue replacement. Establishing hallmarks of the native myocardium in engineered cardiac tissue is a central goal and appears to be required for creating functional tissue that can serve as a surrogate for in vitro testing or the eventual replacement of diseased or injured myocardium. The objective of this research was to apply an engineering approach to develop tools and methods to produce engineered cardiac tissue and characterize both native and engineered cardiac tissue. Three phases of research included: 1) the development and utilization of a framework to characterize microstructure in living cardiac tissue using confocal microscopy and local dye delivery, 2) the development a next-generation bioreactor capable of continuously monitoring force-displacement in engineered tissue, and 3) the application of confocal imaging and image analysis to quantitatively describe features of the native myocardium, focusing on myocyte geometry and spatial distribution of a major gap junction protein connexin-43, in both engineered tissue and native tissue.
Type Text
Publisher University of Utah
Subject Bioreactor; Cardiac tissue structure; Confocal microscopy; Tissue engineering; Heart -- Histology
Dissertation Institution University of Utah
Dissertation Name Doctor of Philosophy
Language eng
Rights Management Copyright © Richard Allen Lasher 2012
Format Medium application/pdf
Format Extent 4,269,311 bytes
Identifier us-etd3,87335
Source Original housed in Marriott Library Special Collections, QM5.5 2012.L37
ARK ark:/87278/s6xp7krb
Setname ir_etd
Date Created 2012-11-16
Date Modified 2021-05-06
ID 195637
Reference URL