The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly

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Publication Type thesis
School or College School of Medicine
Department Biochemistry
Author Ferris, Elliott Carter
Title The role of Cox20 in Cox2 maturation and cytochrome C oxidase assembly
Date 2011-11
Description Proper assembly of Cytochrome c Oxidase (CcO) is vital to mitochondrial respiration. The metallation and incorporation of CcO subunit 2 (Cox2) are important assembly steps that remain poorly understood. The Cox2 assembly factor Cox20 may provide a unique window on CcO assembly. Cox20 is known to bind Cox2 before the latter is incorporated into the larger CcO assembly in S. cerevisiae. Conservation of Cox20 in higher organisms and the lethality of its deletion in Drosophila melanogaster may suggest Cox20 plays a conserved role in CcO assembly. In an attempt to understand the nature of the hypothesized conserved role of Cox20, conserved residues within Cox20 were mutated and the resulting mutants studied. Mutation of a conserved cysteine pair impacted mitochondrial respiration, but did not block CcO assembly. The mutant Cox20 C87A forms a mixed disulfide species. Identifying the partner molecule may shed light on the role of Cox20. The present study finds Cox20, a 23 kD protein, in high molecular weight complexes that are unlikely to be homo-oligomeric. Identifying any other proteins in these complexes may shed light on the role Cox20 plays in CcO assembly. To this end, Cox20 was purified and analyzed by mass spectrometry (MS) to identify any accompanying proteins. Small quantities of the known CcO assembly factors Mss2, Coa1, and Mss51 were identified.
Type Text
Publisher University of Utah
Subject Cox2; Cox20; Cyclooxygenase 2; Cyclooxygenases; Cytochrome oxidase; C oxidase assembly; Mitochondrial respiration; Cox2 assembly factor
Dissertation Institution University of Utah
Dissertation Name Master of Science
Language eng
Rights Management Copyright © Elliott Carter Ferris 2011
Format Medium application/pdf
Format Extent 1,918,461 bytes
Identifier us-etd3,78169
Source Original housed in Marriott Library Special Collections, QP6.5 2011 .F47
ARK ark:/87278/s6g4551n
Setname ir_etd
Date Created 2012-04-24
Date Modified 2021-05-06
ID 194430
Reference URL
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