Dissecting the Role of ALP-1 and EAT-1 Proteins in Regulation of Muscle Structure and Function

Update item information
Publication Type dissertation
School or College School of Medicine
Department Oncological Sciences
Author Han, Hsiao-Fen
Title Dissecting the Role of ALP-1 and EAT-1 Proteins in Regulation of Muscle Structure and Function
Date 2010-04-21
Description The purpose of this dissertation is to define how muscle cells maintain their contractile machinery and how a rhythmic muscle contraction is regulated. These studies employed C. elegans as a model system to characterize pathways required for normal muscle structure and function. With easy manipulation and a wide variety of genetic tools, C. elegans is a powerful and efficient system to study gene function and to dissect underlying molecular mechanisms. In the first part of this dissertation, Z-disc-associated muscle proteins of the ALPEnigma family were characterized. The alp-1 gene in C. elegans encodes multiple ALPEnigma proteins. The ALP-1 proteins are broadly expressed at actin-anchorage sites, and play a role in maintaining actin filament organization within muscles. Genetic analysis revealed that ALP-1 and alpha-actinin act together to support the integrity of actin cytoarchitecture. The second part of this dissertation is focused on the characterization of the eat-1 gene in C. elegans. Mutations in eat-1 cause defects in the rhythmic contraction of the pharynx, an organ in the C. elegans digestive tract that is comprised of muscle, neuronal and epithelial cells, eat-1 encodes adenosine kinase, an evolutionarily conserved enzyme that catalyzes adenosine to adenosine monophosphate. Molecular and genetic studies showed that EAT-1 acts noncell autonomously and modulates adenosine receptormediated signaling to regulate pharyngeal muscle contraction. The studies reveal the important role of ALP-Enigma proteins in maintaining muscle structural integrity, and of adenosine kinase in regulating rhythmic muscle contraction, enhancing our knowledge of how muscle cells maintain their structure and contract properly.
Type Text
Publisher University of Utah
Subject Muscle Contraction; Muscle Proteins; Caenorhabditis elegans
Subject MESH Muscle Contraction; Muscle Proteins; Caenorhabditis elegans Proteins
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Dissecting the role of ALP-1 and EAT-1 proteins in regulation of muscle structure and function." Spencer S. Eccles Health Sciences Library. Print version of "Dissecting the role of ALP-1 and EAT-1 proteins in regulation of muscle structure and function." available at J. Willard Marriot Library Special Collection. QP6.5 2009.H25.
Rights Management © Hsiao-Fen Han
Format Medium application/pdf
Format Extent 2,104,895 bytes
Source Original: University of Utah Spencer S. Eccles Health Sciences Library
Conversion Specifications Original scanned on Fujitsi fi-5220G as 400 dpi to pdf using ABBYY FineReader 10
ARK ark:/87278/s69g627c
Setname ir_etd
Date Created 2012-04-23
Date Modified 2012-04-23
ID 192067
Reference URL https://collections.lib.utah.edu/ark:/87278/s69g627c