Glycosaminoglycans and their binding proteins: biochemical studies and development of potential wound healing biomaterials

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Publication Type dissertation
School or College College of Pharmacy
Department Medicinal Chemistry
Author Cai, Shenshen
Title Glycosaminoglycans and their binding proteins: biochemical studies and development of potential wound healing biomaterials
Date 2005-12
Description Glycosaminoglycans (GAGS), including hyaluronic acid (HA), heparin, heparan sulfate (HS), chondroitin sulfate (CS), dermatan sulfate (DS), and keratan sulfate (KS) are natural polysaccharides widely distributed in the extracellular matrix (ECM), cell surface, and the basement membrane. GAGs bind numerous proteins, which integrate them into many essential biological pathways. Therefore, studying GAGs and their binding proteins is important for the application of GAGs in biotechnology, pharmaceutical, and medical fields. This dissertation mainly discusses HA and heparin, with some biochemical studies with their binding proteins and potential applications to develop wound-healing biomaterials. An HA binding protein, RHAMM (receptor of HA-mediated motility), was engineered to generate a recombinant protein (HB3) with excellent heparin affinity and specificity. Therapeutically relevant heparin (both unfractionated and low molecular weight) can be measured using HB3 protein in a competition assay modified from an enzyme-linked immunosorbent assay (ELISA). This heparin assay has advantages in high consistency and low cost over current methods of activated partial thromboplastin time (APTT) and anti-Xa assays. Another important HA binding protein, SPACRCAN (sialoproteoglycan associated with cones and rods) in retinal areas, was found to bind with heparin and CS. The binding domain was identified to be RHAMM-like BX<sub>7
Type Text
Publisher University of Utah
Subject MESH Glycosaminoglycans; Protein Binding; Sulfates; Heparin
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Glycosaminoglycans and their binding proteins: biochemical studies and development of potential wound healing biomaterials". Spencer S. Eccles Health Sciences Library.
Rights Management © Shenshen Cai.
Format Medium application/pdf
Format Extent 6,920,832 bytes
Identifier undthes,4160
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available)
Master File Extent 6,920,887 bytes
ARK ark:/87278/s6n018fx
Setname ir_etd
ID 191763
Reference URL https://collections.lib.utah.edu/ark:/87278/s6n018fx
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