Isolation and characterization of ecdysone-inducible genes from Drosophila

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Publication Type dissertation
School or College School of Medicine
Department Human Genetics
Author Hurban, Patrick
Contributor Palazzolo, Mike; Thummel, C.S.
Title Isolation and characterization of ecdysone-inducible genes from Drosophila
Date 1994-06
Description Metamorphosis in Drosophila melanogaster is initiated by a pulse of the steroid hormone ecdysone. The genetic response to ecdysone can be visualized in the reproducible puffing patterns of the giant salivary gland polytene chromosomes of late third instar larvae. A hierarchical model to account for salivary gland puffing posits that: ecdysone exerts its effects by reversibly binding to its cognate receptor; the hormone-receptor complex functions both to activate an early set of puffs and to repress a late set of puffs; and products of the early puffs antagonize the activity of the hormone-receptor complex by activating the late puffs and repressing the early puffs. In an effort to extend this regulatory hierarchy beyond the salivary glands, a subtracted cDNA library was constructed that is enriched for genes directly induced by ecdysone in cultured larval organs. Ecdysone-inducible genes (Eigs) were identified by testing individual cDNAs from the subtracted library for their ability to detect ecdysone-inducible transcripts in cultured larval organs. Cytological mapping of the Eigs led to the identification of two previously characterized early puff genes, E75 and E63-2. The temporal patterns of Eig expression in the organ culture system and in whole animals was determined by RNA blot analysis. Tissue-specific patterns of Eig expression were determined by in situ hybridization to cryostat sections of staged larvae and prepupae. Sequence analysis of Eig cDNAs led to the identification of a novel cytochrome P450 gene and a novel C3HC4 type zinc-finger protein.
Type Text
Publisher University of Utah
Subject Zinc Fingers; Cytochrome P-450; Molecular Biology
Subject MESH Drosophila melanogaster; Genetics; Ecdysone
Dissertation Institution University of Utah
Dissertation Name PhD
Language eng
Relation is Version of Digital reproduction of "Isolation and characterization of ecdysone-inducible genes from Drosophila." Spencer S. Eccles Health Sciences Library. Print version of "Isolation and characterization of ecdysone-inducible genes from Drosophila." available at J. Willard Marriott Library Special Collection. QL3.5 1994 .H87.
Rights Management © Patrick Hurban.
Format Medium application/pdf
Format Extent 4,337,596 bytes
Identifier undthes,5451
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Master File Extent 4,337,640 bytes
ARK ark:/87278/s6r2137s
Setname ir_etd
Date Created 2012-04-24
Date Modified 2021-05-06
ID 191530
Reference URL
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