Investigation of 5' messenger RNA stability determinants in lacZ

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Publication Type dissertation
School or College School of Medicine
Department Human Genetics
Author Wagner, Lori Ann
Title Investigation of 5' messenger RNA stability determinants in lacZ
Date 1994-03
Description A series of initiation codon and Shine-Dalgarno (SD) sequence changes shows that the SD but not the initiation codon is necessary to maintain lacZ mRNA stability in Escherichia coli. A point mutation in the 4th codon (transcript base 55) of lacZ decreases mRNA stability and beta-galactosidase activity. This mutation also causes decreased beta-galactosidase activity in the background of altered SD and initiation codons. However, mRNA stability is not decreased in the presence of base 55-C and altered initiation codons. Downstream lacZ sequence up to codon 62 is also important for base 55-C's effect on gene expression. Therefore, ribosomal binding but not translation of the coding region is thought to be required for lacZ mRNA stability.
Type Text
Publisher University of Utah
Subject Bacterial Genetics; Proteins
Subject MESH RNA, Messenger; Escherichia coli
Dissertation Institution University of Utah
Dissertation Name PhD
Language en
Relation is Version of Digital reproduction of “An investigation of 5' messenger RNA stability determinants in lacZ.” Spencer S. Eccles Health Sciences Library. Print version of “An investigation of 5' messenger RNA stability determinants in lacZ.” available at J. Willard Marriott Library Special Collection. QP6.5 1994 .W33.
Rights Management © Lori Ann Wagner.
Format Medium application/pdf
Format Extent 4,460,213 bytes
Identifier undthes,5119
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship DOE grant DEFG 88ER60700 and Howard Huges Medical Institute.
Master File Extent 4,460,246 bytes
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CONTENTdm URL http://cdmbuntu.lib.utah.edu/cdm/ref/collection/etd1/id/993