Phosphatase activity in normal and virus infected tissue culture systems

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Publication Type thesis
School or College School of Medicine
Department Pathology
Author Deig, Elmer Frank
Title Phosphatase activity in normal and virus infected tissue culture systems
Date 1958-08
Description Non-specific alkaline phosphatase, ATP-ase, and glucose-6-phosphatase activities were assayed in intact tissue cultured cell from monkey kidneys with suitable substrate-buffer systems. The results were expressed in terms of released orthophosphate, over a give time, from cells in contrast with the sodium salts of glycerophosphate, ATP, or glucose-6-phosphate. The reproducibility of results was verified in each experiment in multiple analysis on a number of cell monolayers treated in an identical fashion. The effect of substrate and substrate concentration, pH, Ca++ and Mg++ concentration, glucose and L-glutamine, maleic acid, and 2,4-dinitrophenol on certain of these enzyme activities was determined. Possible variations in enzymatic activity between normal and poliovirus infected cells were noted, and the capacity of the cells to synthesize virus was measured under each of the assay conditions used. Factors which interfered with normal viral synthesis were reproduced with cells in a nutritionally complete medium, in certain instance, to see if the original interference still persisted, or to find whether the amount of virus produce in a given time was altered from normal tissue culture condition. The results from these experiments are summarized in the Discussion of this thesis.
Type Text
Publisher University of Utah
Subject Glucose; Morphology
Subject MESH Phosphoric Monoester Hydrolases; Metabolism; Enzyme Activation
Dissertation Institution University of Utah
Dissertation Name MS
Language eng
Relation is Version of Digital reproduction of "Phosphatase activity in normal and virus infected tissue culture systems." Spencer S. Eccles Health Sciences Library. Print version of "Phosphatase activity in normal and virus infected tissue culture systems." available at J. Willard Marriott Library Special Collection. QP6.5 1958 .D44.
Rights Management © Elmer Frank Deig.
Format Medium application/pdf
Format Extent 3,181,034 bytes
Identifier undthes,5315
Source Original: University of Utah Spencer S. Eccles Health Sciences Library (no longer available).
Funding/Fellowship Grant from The National Foundation for Infantile Paralysis.
Master File Extent 3,181,097 bytes
ARK ark:/87278/s6n58p5p
Setname ir_etd
Date Created 2012-04-24
Date Modified 2012-04-24
ID 191136
Reference URL https://collections.lib.utah.edu/ark:/87278/s6n58p5p