Quantitative Assessment of Normal and Hyperthyroid Extraocular Muscles in C57BL/6 Mice Using Confocal Laser Scanning Microscopy

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Identifier 20070213_nanos_sciplatform_05.pdf
Title Quantitative Assessment of Normal and Hyperthyroid Extraocular Muscles in C57BL/6 Mice Using Confocal Laser Scanning Microscopy
Creator Bose, Swaraj; Nien, Chyong; Kenney, Cristina; Wallace, Douglas; Jester, James
Subject Confocal Microscopy; Thyroid eye disease; Extraocular muscles; Mitochondria; Experimental Hyperthyroidism
Description The study aims to determine if confocal laser scanning microscopy (CLSM) is able to distinguish structural and functional changes in extraocular muscles (EOMs) of hyperthyroid mice known to differ from normal controls. We employed CLSM on EOMs using two-photon excited fluorescence (TPEF), second-harmonic image microscopy (SHIM), mitotracker, JC-1 and FITC-phalloidin fluorescence staining to quantify and localize myofibrils, mitochondria, and actin-myosin complex abnormalities of EOMs.
Language eng
Format application/pdf
Format Creation application/pdf
Type Text
Source 2007 North American Neuro-Ophthalmology Society Annual Meeting
Relation is Part of NANOS 2007: Scientific Platform Presentations (Session II)
Collection Neuro-ophthalmology Virtual Education Library: NOVEL http://NOVEL.utah.edu
Publisher Spencer S. Eccles Health Sciences Library, University of Utah
Holding Institution North American Neuro-Ophthalmology Association. NANOS Executive Office 5841 Cedar Lake Road, Suite 204, Minneapolis, MN 55416
Rights Management Copyright 2010. For further information regarding the rights to this collection, please visit: https://NOVEL.utah.edu/about/copyright
ARK ark:/87278/s65j0npd
Context URL The NANOS Annual Meeting Neuro-Ophthalmology Collection: https://novel.utah.edu/collection/NAM/toc/
Setname ehsl_novel_nam
ID 180941
Reference URL https://collections.lib.utah.edu/ark:/87278/s65j0npd
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