Exploring adipose biology in zebrafish

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Publication Type thesis
School or College School of Medicine
Department Biochemistry
Author Aryal, Madhukar
Title Exploring adipose biology in zebrafish
Date 2014-12
Description Adipose tissue plays a key role in metabolic homeostasis. Adipose tissue functions are associated with various pathological conditions including obesity, insulin resistance, and other metabolic complications. Understanding the normal regulation of adipose tissue formation and homeostasis is imperative to understanding the pathologies associated with a decrease or increase in adipose tissue. We have undertaken an unbiased forward genetic screening to identify novel genes that affect lipid storage in zebrafish adipose tissue. We aimed to clone and characterize gene(s) responsible for sans suet (sns) mutant. We utilized next generation sequencing analysis and obtained four candidate variants that may have genetic linkage with sns mutation. The targeted sequencing revealed that the candidate variant on chromosome 5 (hdr gene) was heterozygous in sns mutant fish. We hypothesize that the region near the hdr gene may have a stretch of a homozygous region that may be linked to sns phenotype. In addition, our goal was to develop transgenic reporter and molecular tools to manipulate gene function for the study of adipose biology in zebrafish. We aimed to create a transgenic reporter line that expresses mCherry exclusively in adipocytes. While testing the nuclease activity of Transcription Activator Like-Effector Nuclease (TALEN) in vivo, we found the inefficient introduction of double strand breaks in the predefined site of the genome. This result necessitates redesigning the genome engineering nuclease for efficient double strand breakage for successful knock-in.
Type Text
Publisher University of Utah
Subject MESH Adipose Tissue; Adipose Tissue, White; Lipoproteins; Fatty Acids; Homeostasis; Zebrafish; Adipogenesis; Metamorphosis, Biological; Transcription Activator-Like Effector Nucleases; Whole Exome Sequencing; Genome; Lipid Metabolism; Animals, Genetically Modified; Enhancer Elements, Genetic; Gene Expression Regulation; Gene Transfer Techniques; Polymorphism, Genetic; DNA Primers
Dissertation Institution University of Utah
Dissertation Name Master of Science
Language eng
Relation is Version of Digital reproduction of Exploring Adipose Biology in Zebrafish
Rights Management Copyright © Madhukar Aryal 2014
Format Medium application/pdf
Format Extent 954,404 bytes
Source Original in Marriott Library Special Collections
ARK ark:/87278/s67t2339
Setname ir_etd
ID 1422283
Reference URL https://collections.lib.utah.edu/ark:/87278/s67t2339
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